Microbiology
5'...A^G A T C T...3'rn3'...T C T A G^A...5'rnrnSupplied with: 10X OPTIZYME Buffer 3rnConditions for 100% Activity:rnrn1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSArnIncubate at 37°CrnEnzyme Activity in OPTIZYME buffers:rnBuffer 1: 0 - 20%rnBuffer 2: 20 - 50%rnBuffer 3: 100%rnBuffer 4: 0 - 20%rnBuffer 5: 50 - 100%rnStorage Buffer:rn10mM Tris-HCl (pH 8.2 at 25°C), 200 mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycerolrnLigation and Re-cleavage:rnMore than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with BglII.rnDigestion of Agarose-embedded DNA:rnA minimum of 5 units of BglII is required for the complete digestion of 1µg of agarose-embedded lambda DNA in 16 hours.rnCompatible Ends: BamHI, BclI, Bsp143I, MboI, PsuIrnMethylation Effects:rnDam: Completely overlaps - no effectrnAGm6ATCTrn(No effect on cleavage)rnDcm: Never overlaps - no effectrnCpG: Never overlaps - no effectrnEcoKI: Never overlaps - no effectrnEcoBI: May overlap - cleavage impairedrn5'...TGm6AGATCT(N)3 TGCT...3'rn3'...AC TCTAGA(N)3m6ACGA...5'rn(Cleavage is impaired)
| Item Number | FSBP8014-5 |
| Packaging | Each |
| Brochure |
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