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Laboratory » Microbiology

25,000U OPTIZYME™ M-MLV Reverse Transcriptase, Fisher BioReagents™
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Manufacturer: Fisher Scientific
Item#: FSBP81045

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Product Description

OPTIZYME™ M-MuLV RT is a genetically modified MLV RT that possesses RNA- and DNA-dependent polymerase activity.rnrnApplications and Features:rnrnOPTIZYME M-MLV RT is used for efficient first strand cDNA synthesis for RT-PCR and real-time RT-PCR (up to 13kb)rnGeneration of labeled cDNA probes for microarraysrnSynthesis of cDNA for cloning and expressionrnAnalysis of RNA by primer extensionrnDNA labelingrnOptimum activity at 42°C (active up to 50°C)rnIncorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)rnSupplied with: rn5X OPTIZYME M-MLV RT Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 20mM MgCl2, 50mM DTTrnSource: E. Coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptasernQuality Control:rnrnEndo-, exodeoxyribonuclease, phosphatase and ribonuclease-freernFunctionally tested in first strand cDNA and RT-PCR

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Item Number FSBP81045
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