Microbiology
5'...C^T N A G...3'rn3'...G A N T^C...5'rnrnSupplied with: 10X OPTIZYME Buffer 4rnConditions for 100% Activity:rnrn1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSArnIncubate at 37°CrnEnzyme Activity in OPTIZYME buffers:rnBuffer 1: 20 - 50%rnBuffer 2: 20 - 50%rnBuffer 3: 20 - 50%rnBuffer 4: 100%rnBuffer 5: 20 - 50%rnrnStorage Buffer:rn10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerornlrnLigation and Re-cleavage:rnMore than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI.rnCompatible Ends: BbvCI, Bpu10I, Bpu1102I, Eco81IrnMethylation Effects:rnDam: Never overlaps - no effectrnDcm: Never overlaps - no effectrnCpG: Never overlaps - no effectrnEcoKI: Never overlaps - no effectrnEcoBI: May overlap - effect not determined
| Item Number | FSBP8060-5 |
| Packaging | Each |
| Brochure |
 Click here to download
|
